Purification of arginase from Aspergillus nidulans.
نویسندگان
چکیده
منابع مشابه
Purification and characterization of phosphatase I from Aspergillus nidulans.
Repressible alkaline phosphatases have been reported to occur in a variety of microorganisms, including Es&e&& coli (1, 2), Staphylococcus aureus (3)) Bacillus subtilis (4)) Neurospora crassa (5), and baker’s yeast (6). Under conditions of limiting phosphate, the fungus Aspergillus nidulans produces substantial amounts of four electrophoretically distinct phosphomonesterases (7). Two of these e...
متن کاملPurification and Characterization of Phosphatase I from Aspergillus nidulans*
Repressible alkaline phosphatases have been reported to occur in a variety of microorganisms, including Es&e&& coli (1, 2), Staphylococcus aureus (3)) Bacillus subtilis (4)) Neurospora crassa (5), and baker’s yeast (6). Under conditions of limiting phosphate, the fungus Aspergillus nidulans produces substantial amounts of four electrophoretically distinct phosphomonesterases (7). Two of these e...
متن کاملPurification and preliminary characterization of alcohol dehydrogenase from Aspergillus nidulans.
Aspergillus alcohol dehydrogenase is produced in response to growth in the presence of a wide variety of inducers, of which the most effective are short-chain alcohols and ketones, e.g. butan-2-one and propan-2-ol. The enzyme can be readily extracted from fresh or freeze-dried cells and purified to homogeneity on Blue Sepharose in a single step by using specific elution with NAD+ and pyrazole. ...
متن کاملAspergillus nidulans
Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...
متن کاملIsolation of nuclei from Aspergillus nidulans protoplasts.
Intact nuclei were isolated from the protoplasts of the filamentous fungus Aspergillus nidulans. The large amounts of protoplasts required for such nuclear preparations were produced from young mycelia grown in liquid culture. For final purification of the crude nuclear fraction, a Nycodenz density-gradient centrifugation was applied. The resulting nuclei were of good purity and morphological s...
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ژورنال
عنوان ژورنال: Acta Biochimica Polonica
سال: 1994
ISSN: 1734-154X,0001-527X
DOI: 10.18388/abp.1994_4700